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Wendy Gilbert Professor wgilbert /at\ mit /d0t\ edu |
Audra Amasino Graduate Student amasino /at\ mit /d0t\ edu |
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Joshua Arribere Graduate Student arribere /at\ mit /d0t\ edu |
| Distinct transcript leaders (5'TLs) vary by orders of magnitude in the translational efficiencies they impart on the downstream ORF, and these affects change as a function of the physiological state of the cell. To study this, I am first developing genome-wide assays for 5'TL identification and quantification. Subsequently I will work towards an understanding of the cis- and trans-acting factors acting via 5'TLs which account for the great variation in protein synthesis rates amongst ORFs. |
Kristen Bartoli Post-doctoral Researcher kristen.bartoli /at\ gmail /d0t\ com |
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Thomas Carlile Post-doctoral Researcher tcarlile /at\ mit /d0t\ edu |
Maria Fernanda Research Scientist mfrd18 /at\ gmail /d0t\ com |
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Mary Kay Thompson Graduate Student marykayt /at\ mit /d0t\ edu |
Pavan Vaidyanathan Post-doctoral Researcher pavanpv /at\ mit /d0t\ edu |
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| Although ribosomes are currently thought to be unchanging protein factories capable of translating all cellular messages, accumulating evidence suggests that ribosomes may be subject to extensive regulation by numerous mechanisms depending on the specific cellular condition. Using glucose starved yeast cells as a model, I am exploring the hypothesis that functionally specialized ribosomes capable of translating only specific sets of messages are produced for specific environmental stresses and conditions. Additionally, I am also attempting to understand the role of non-ribosomal accessory proteins in this specialization process by looking at the translational consequences of the over-expression of one such accessory protein. |
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Boris Zinshteyn Graduate Student borisz /at\ mit /d0t\ edu |
| Transfer RNAs (tRNAs) from all domains of life contain numerous post-transcriptional modifications, many of which are highly conserved, yet the molecular and physiological functions of many of these modifications are unknown. I am using genomic and genetic approaches to identify the translational defects associated with loss of specific anticodon modifications in yeast. |
Former Lab Members
Joel Greenwood Whiz Kid greenwood.joel /at\ gmail /d0t\ com |
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Jacqueline Nkuebe Undergraduate Student jnnkuebe /at\ gmail /d0t\ com |
Krithika Shanmugasundaram Undergraduate Student krithika.shanmugasundaram /at\ mit /d0t\ edu |
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Jabe Ziino Undergraduate Student jabe /at\ mit /d0t\ edu |
Ekatarina Zagriadskaia Senior Research Scientist zagriads /at\ mit /d0t\ edu |
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